ovarian cancer cell lines es2  (ATCC)

Journal: Genome medicine

Article Title: Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy.

doi: 10.1186/s13073-025-01490-0

Figure Lengend Snippet: Fig. 6 PVRbbz CAR-T cells effectively killed multiple tumor cell lines even at low effector:target ratios and corroborated by cytokines release. A-L Cytotoxicity of PVRbbz CAR-T cells on PC3 (A and G), PANC1 (B and H), U20S (C and I), ES2 (D and J), Huh7 (E and K) and MM.1S (F and L) cells at the indicated effector:target (E:T) ratios after 12 h of co-culturing. Cell Lysis was determined by luciferase and relative IVIS picture was shown. Two independent experiments were performed. M and N The concentrations of TNF-α (M) and IFN-γ (N) in supernatants from cytotoxicity assays at 1:1 effector:target after co-culture with tumor cells for 12 h were measured by ELISA kits. Three independent experiments were performed. Significance was calculated by two-way ANOVA. All error bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: Prostate cancer cell lines PC3, C4-2, DU145, multiple myeloma MM.1S cell line, chronic myeloid leukemia K562 cell line, osteosarcoma cell lines U20S and 143B, ovarian cancer cell line ES2 and OVCAR8, pancreatic cancer cell line PANC1 and CAPAN-2, lung cancer cell line H292, colorectal carcinoma cell line HT29 and murine melanoma cell line B16 F10 were obtained from the American Type Culture Collection (ATCC).

Techniques: Lysis, Luciferase, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

Journal: Molecular biology reports

Article Title: Inhibiting eukaryotic initiation factor 5A (eIF5A) hypusination attenuated activation of the SIK2 (salt-inducible kinase 2)-p4E-BP1 pathway involved in ovarian cancer cell proliferation and migration.

doi: 10.1007/s11033-023-08510-5

Figure Lengend Snippet: Fig. 1 Depletion of eIF5AHyp reduces the activity of a luciferase reporter gene containing a repeated proline motif and reduces endog- enous SIK2 protein level. A Schematic diagram of the reporter constructs with a repeated proline motif (F-pro-FLuc-WT) and the mutant control reporter (F-pro-FLuc-Mut) in FLAG vector. B Fire- fly luciferase activity assay following GC7 or eIF5A-targeting siRNA treatment in the presence of luciferase reporters constructed with repeated proline motifs. ES2 cell lines were transfected with a F-pro- FLuc-WT vector or its mutant vector by electroporation. After 12 h, the transfected ES2 cells were treated with GC7 or eIF5A-targeting siRNA, further incubated for 36–72 h, and harvested to determine the luciferase activity. The firefly luciferase level was normalized to the Renilla luciferase level. Data are mean ± SE of triplicate experiments using GC7 or eIF5A-targeting siRNA. C Using western blotting, the levels of eIF5AHyp, total eIF5A, total SIK2, and β-actin in ES2 cells

Article Snippet: The TOV-112D, CAOV-3, OVCAR-3, and ES2 human ovarian cancer cell lines were purchased from ATCC (Manassas, VA).

Techniques: Activity Assay, Luciferase, Construct, Mutagenesis, Control, Plasmid Preparation, Transfection, Electroporation, Incubation, Western Blot

Journal: Molecular biology reports

Article Title: Inhibiting eukaryotic initiation factor 5A (eIF5A) hypusination attenuated activation of the SIK2 (salt-inducible kinase 2)-p4E-BP1 pathway involved in ovarian cancer cell proliferation and migration.

doi: 10.1007/s11033-023-08510-5

Figure Lengend Snippet: Fig. 2 Validation of p4E-BP1(Ser 65) as SIK2-associated protein. A Cell lysates were prepared from ES2 cells transfected with FLAG- tagged SIK2 and FLAG-EV. A pull-down assay was performed with anti-FLAG agarose beads. The pulled-down samples and unbound fractions were subjected to 4–12% NuPAGE gel and visualized by Ponceau S staining. The pulled-down proteins were analyzed by west- ern blotting with the indicated antibody. Total SIK2, total 4E-BP1, and p4E-BP1(Ser 65) proteins and their molecular weights (MWs) are shown on the right. B ES2 cells were transfected with siRNA to silence the SIK2 or 4E-BP1 genes and harvested 72 h after trans- fection; the cell lysates were analyzed by western blotting with the indicated antibody. The total SIK2 and p4E-BP1(Ser 65) proteins and their MWs are shown on the right. C ES2 cells were transfected with FLAG-EV and FLAG-tagged SIK2 in the absence or presence of GC7. After 12 h, the transfected ES2 cells were treated with GC7

Article Snippet: The TOV-112D, CAOV-3, OVCAR-3, and ES2 human ovarian cancer cell lines were purchased from ATCC (Manassas, VA).

Techniques: Biomarker Discovery, Transfection, Pull Down Assay, Staining, Western Blot

Journal: Molecular biology reports

Article Title: Inhibiting eukaryotic initiation factor 5A (eIF5A) hypusination attenuated activation of the SIK2 (salt-inducible kinase 2)-p4E-BP1 pathway involved in ovarian cancer cell proliferation and migration.

doi: 10.1007/s11033-023-08510-5

Figure Lengend Snippet: Fig. 3 Depletion of eIF5AHyp reduces the activity of the SIK2-p4E- BP1(Ser 65) pathway, which is involved in cell proliferation and migration. ES2 cells prepared by GC7 treatment or siRNA transfec- tion for eIF5A gene silencing and siRNA transfection for SIK2 or 4E-BP1 gene silencing were grown in 96-well, 6-well, and 12-well culture plates and cultured for 36–72 h. In addition, ES2 cells were transfected with FLAG-EV, FLAG-tagged SIK2, or FLAG-tagged 4E-BP1 in the absence or presence of GC7. After 12 h, the trans- fected ES2 cells were treated with GC7 (50 μM) and further cultured for 36 h. A Representative images and quantification of wound heal- ing in ES2 cell monolayers. The relative percentages of wound area

Article Snippet: The TOV-112D, CAOV-3, OVCAR-3, and ES2 human ovarian cancer cell lines were purchased from ATCC (Manassas, VA).

Techniques: Activity Assay, Migration, Transfection, Cell Culture